A genetically-encoded photosensitiser demonstrates killing of bacteria by purely endogenous singlet oxygen.
نویسندگان
چکیده
TagRFP, a fluorescent protein capable of photosensitizing the production of singlet oxygen, was expressed in E. coli. Subsequent exposure to green light induced bacterial cell death in a light-dose dependent manner. It is demonstrated for the first time that intracellular singlet oxygen is sufficient to kill bacteria.
منابع مشابه
Water soluble quantum dots as hydrophilic carriers and two-photon excited energy donors in photodynamic therapy†
In search of strategies to develop deeply penetrating agents for use in Photodynamic Therapy (PDT), we have devised a Quantum Dot-Rose Bengal conjugate that is effective at producing singlet oxygen upon two-photon irradiation. The CdSe/ZnS Quantum Dot, with its high two photon absorption cross section, serves as a two-photon absorbing antenna and transfers its excited state energy to the attach...
متن کاملComparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen.
Gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. Killing curves for Salmonella typhimurium and Escherichia coli strains were indicative of multihit killing, whereas curves for Sarcina lutea, Staphylococcus aureus, Streptococcus lactis, and Streptococcus faecalis exhibited single-hit kinetics. The S. typhimu...
متن کاملComparative study of singlet oxygen production by photosensitiser dyes encapsulated in silicone: towards rational design of anti-microbial surfaces.
Surfaces with built-in antimicrobial activity have the potential to reduce hospital-acquired infections. One promising strategy is to create functionalised surfaces which, following illumination with visible light, are able to generate singlet oxygen under aerobic conditions. In contrast to antibiotics, the mechanism of bacterial kill by species derived from reactions with singlet oxygen is com...
متن کاملThe hydroxypyridinone iron chelator CP94 increases methyl-aminolevulinate-based photodynamic cell killing by increasing the generation of reactive oxygen species
Methyl-aminolevulinate-based photodynamic therapy (MAL-PDT) is utilised clinically for the treatment of non-melanoma skin cancers and pre-cancers and the hydroxypyridinone iron chelator, CP94, has successfully been demonstrated to increase MAL-PDT efficacy in an initial clinical pilot study. However, the biochemical and photochemical processes leading to CP94-enhanced photodynamic cell death, b...
متن کاملDispersal patterns of endogenous bacteria among grass carp (Ctenopharyngodon idellus) guts
The formation and regulation of vertebrate endogenous intestinal microbiota has been widely studied as the microbiota plays a crucial role in the host nutrition, development, and health. Despite the importance of microbiota for host health, it is still unclear whether the endogenous intestinal microorganisms are genetically distinct or whether they are genetically related with each other in dif...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
دوره 11 9 شماره
صفحات -
تاریخ انتشار 2012